Dietary docosahexaenoic acid-enriched glycerophospholipids exert cardioprotective effects in ouabain-treated rats via physiological and metabolic changes.
Identifieur interne : 000072 ( Main/Exploration ); précédent : 000071; suivant : 000073Dietary docosahexaenoic acid-enriched glycerophospholipids exert cardioprotective effects in ouabain-treated rats via physiological and metabolic changes.
Auteurs : Monique Bernard [France] ; Jean-Michel Maixent [France] ; Alain Gerbi [France] ; Carole Lan [France] ; Patrick Jean Cozzone [France] ; Gérard Pieroni [France] ; Martine Armand [France] ; Thierry Charles Coste [France]Source :
- Food & function [ 2042-650X ] ; 2016.
Abstract
Docosahexaenoic acid (DHA) might prevent heart failure or optimise drug treatments by improving cardiac contraction. We investigated whether DHA-enriched avian glycerophospholipids (GPL-DHA) exert cardioprotection in ouabain-treated rats after 4 weeks of dietary supplementation with 10, 35 or 60 mg DHA per kg body weight versus none (DHA10, DHA35, DHA60 and control groups, respectively). The contractile responsiveness to different doses of ouabain (10(-7) to 10(-4) M), ouabain intoxication (at 3 × 10(-4) M), and relative variations in cardiac energy metabolism were determined using (31)P NMR in isolated perfused rat hearts. The fatty acid composition of cardiac membranes was analysed by gas chromatography. DHA accretion in the heart was dose-dependent (+8%, +30% and +45% for DHA10, DHA35 and DHA60, respectively). The cardiac phosphocreatine content significantly increased at the baseline in DHA35 (+45%) and DHA60 groups (+85%), and at the different doses of ouabain in the DHA60 group (+73% to 98%). The maximum positive inotropy achieved at 10(-4) M ouabain was significantly increased in all DHA groups versus control (+150%, +122.5% and +135% for DHA10, DHA35 and DHA60, respectively), and ouabain intoxication was delayed. The increase in myocardial phosphocreatine content and the improved efficacy of ouabain on myocardial contraction without toxicity suggest the potential of GPL-DHA as a dietary supplement or ingredient for functional food, and possibly as a co-treatment with digitalis drugs in humans.
DOI: 10.1039/c5fo01300c
PubMed: 26662260
Affiliations:
- France
- Auvergne (région administrative), Auvergne-Rhône-Alpes, Haute-Normandie, Provence-Alpes-Côte d'Azur, Région Normandie
- Etrépagny, Hauterive, La Garde, Le Havre, Marseille
- Université d'Aix-Marseille
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sort="Maixent, Jean Michel" uniqKey="Maixent J" first="Jean-Michel" last="Maixent">Jean-Michel Maixent</name><affiliation wicri:level="3"><nlm:affiliation>EB2M-Protée, Université du Sud Toulon-Var, F-83957 La Garde, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>EB2M-Protée, Université du Sud Toulon-Var, F-83957 La Garde</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">La Garde</settlement></placeName></affiliation></author><author><name sortKey="Gerbi, Alain" sort="Gerbi, Alain" uniqKey="Gerbi A" first="Alain" last="Gerbi">Alain Gerbi</name><affiliation wicri:level="3"><nlm:affiliation>RDVC Produits Santé, F-76600 Le Havre, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>RDVC Produits Santé, F-76600 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Lan, Carole" sort="Lan, Carole" uniqKey="Lan C" first="Carole" last="Lan">Carole Lan</name><affiliation wicri:level="4"><nlm:affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Cozzone, Patrick Jean" sort="Cozzone, Patrick Jean" uniqKey="Cozzone P" first="Patrick Jean" last="Cozzone">Patrick Jean Cozzone</name><affiliation wicri:level="4"><nlm:affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Pieroni, Gerard" sort="Pieroni, Gerard" uniqKey="Pieroni G" first="Gérard" last="Pieroni">Gérard Pieroni</name><affiliation wicri:level="3"><nlm:affiliation>Application Santé des Lipides, ASL, Bioparc de Vichy, F-03270 Hauterive, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Application Santé des Lipides, ASL, Bioparc de Vichy, F-03270 Hauterive</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Auvergne (région administrative)</region><settlement type="city">Hauterive</settlement></placeName></affiliation></author><author><name sortKey="Armand, Martine" sort="Armand, Martine" uniqKey="Armand M" first="Martine" last="Armand">Martine Armand</name><affiliation wicri:level="4"><nlm:affiliation>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France. monique.bernard@univ-amu.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Coste, Thierry Charles" sort="Coste, Thierry Charles" uniqKey="Coste T" first="Thierry Charles" last="Coste">Thierry Charles Coste</name><affiliation wicri:level="3"><nlm:affiliation>Novastell, F-27150 Etrépagny, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Novastell, F-27150 Etrépagny</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Etrépagny</settlement></placeName></affiliation></author></analytic><series><title level="j">Food & function</title><idno type="eISSN">2042-650X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Docosahexaenoic acid (DHA) might prevent heart failure or optimise drug treatments by improving cardiac contraction. We investigated whether DHA-enriched avian glycerophospholipids (GPL-DHA) exert cardioprotection in ouabain-treated rats after 4 weeks of dietary supplementation with 10, 35 or 60 mg DHA per kg body weight versus none (DHA10, DHA35, DHA60 and control groups, respectively). The contractile responsiveness to different doses of ouabain (10(-7) to 10(-4) M), ouabain intoxication (at 3 × 10(-4) M), and relative variations in cardiac energy metabolism were determined using (31)P NMR in isolated perfused rat hearts. The fatty acid composition of cardiac membranes was analysed by gas chromatography. DHA accretion in the heart was dose-dependent (+8%, +30% and +45% for DHA10, DHA35 and DHA60, respectively). The cardiac phosphocreatine content significantly increased at the baseline in DHA35 (+45%) and DHA60 groups (+85%), and at the different doses of ouabain in the DHA60 group (+73% to 98%). The maximum positive inotropy achieved at 10(-4) M ouabain was significantly increased in all DHA groups versus control (+150%, +122.5% and +135% for DHA10, DHA35 and DHA60, respectively), and ouabain intoxication was delayed. The increase in myocardial phosphocreatine content and the improved efficacy of ouabain on myocardial contraction without toxicity suggest the potential of GPL-DHA as a dietary supplement or ingredient for functional food, and possibly as a co-treatment with digitalis drugs in humans.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne (région administrative)</li><li>Auvergne-Rhône-Alpes</li><li>Haute-Normandie</li><li>Provence-Alpes-Côte d'Azur</li><li>Région Normandie</li></region><settlement><li>Etrépagny</li><li>Hauterive</li><li>La Garde</li><li>Le Havre</li><li>Marseille</li></settlement><orgName><li>Université d'Aix-Marseille</li></orgName></list><tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Bernard, Monique" sort="Bernard, Monique" uniqKey="Bernard M" first="Monique" last="Bernard">Monique Bernard</name></region><name sortKey="Armand, Martine" sort="Armand, Martine" uniqKey="Armand M" first="Martine" last="Armand">Martine Armand</name><name sortKey="Coste, Thierry Charles" sort="Coste, Thierry Charles" uniqKey="Coste T" first="Thierry Charles" last="Coste">Thierry Charles Coste</name><name sortKey="Cozzone, Patrick Jean" sort="Cozzone, Patrick Jean" uniqKey="Cozzone P" first="Patrick Jean" last="Cozzone">Patrick Jean Cozzone</name><name sortKey="Gerbi, Alain" sort="Gerbi, Alain" uniqKey="Gerbi A" first="Alain" last="Gerbi">Alain Gerbi</name><name sortKey="Lan, Carole" sort="Lan, Carole" uniqKey="Lan C" first="Carole" last="Lan">Carole Lan</name><name sortKey="Maixent, Jean Michel" sort="Maixent, Jean Michel" uniqKey="Maixent J" first="Jean-Michel" last="Maixent">Jean-Michel Maixent</name><name sortKey="Pieroni, Gerard" sort="Pieroni, Gerard" uniqKey="Pieroni G" first="Gérard" last="Pieroni">Gérard Pieroni</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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